Fig. 4. IL-10 production is increased in septic AT-treated mice and exogenous IL-10 administration reiterates the amelioration of septic shock and leukocyte response seen with AT treatment. A) IL-10 levels were quantified from peritoneal lavage (PL) six hours after CLP; * p<0.05 vs CLP+NS; n = 10 mice/group. B) Mice were randomized to intraperitoneal delivery of NS or 500 ng recombinant murine IL-10 at the time of CLP. Aural temperature was determined 16 hours post-CLP. * p<0.05 vs CLP+NS; n = 8 mice/group. PL and blood were collected for cellular and cytokine analysis, respectively. C) Serum KC levels and D) local neutrophil accumulation were determined for septic control and IL-10-treated mice three hours after CLP. * p<0.05 vs CLP+NS; n = 16 mice/group. E) Serum MCP-1 levels were determined for septic control and IL-10-treated mice three hours after CLP. * p<0.05 vs CLP+NS; n = 16 mice/group.